Removal of detergent and solvent from solvent-detergent-treated immunoglobulins

The solvent-detergent (S/D) method was applied for inactivation of lipid-en veloped viruses during the production of immunoglobulins. Amberlite XAD-7 r esin was used for removal of solvent (tri-n-butyl phosphate, TnBP) and dete rgent (Triton X-100) after the performed S/D inactivation procedure. The S/ D reagents from the immunoglobulin preparation were adsorbed on Amberlite X AD-7, while immunoglobulins passed through the column and retained their bi ological activity. Using the method developed here, the final immunoglobuli n preparation contains less than 1 ppm of Triton X-100 and less than 2 ppm TnBP. (C) 1999 Elsevier Science B.V. All rights reserved.