High concentrations of sodium chloride facilitate the use of immobilized chymotrypsin for separating virgin forms of specific trypsin inhibitors

It has been shown that specific trypsin inhibitors exhibit also antichymotr ypsin activity in the presence of high NaCl concentrations. Taking advantag e of this phenomenon a simple procedure of separation of the virgin forms o f trypsin inhibitors from squash seeds and porcine pancreas (Kazal) was ela borated. In a typical experiment the inhibitor sample was loaded onto immob ilized chymotrypsin equilibrated with 5 M NaCl at pH 8. After washing out u nadsorbed material the virgin forms of inhibitors could be eluted either wi th water, buffer pH 8.0 or 0.02 M citrate buffer pH 2.6 containing no NaCl. (C) 1999 Elsevier Science B.V. All rights reserved.