A SIMPLE AND EFFECTIVE CHEMILUMINESCENT DNA-FINGERPRINTING TECHNIQUE USING DIGOXIGENIN-LABELED MINISATELLITE PROBES

Citation
Gj. Gissing et Tj. Crease, A SIMPLE AND EFFECTIVE CHEMILUMINESCENT DNA-FINGERPRINTING TECHNIQUE USING DIGOXIGENIN-LABELED MINISATELLITE PROBES, Canadian journal of zoology, 75(5), 1997, pp. 817-820
Citations number
12
Categorie Soggetti
Zoology
Journal title
ISSN journal
00084301
Volume
75
Issue
5
Year of publication
1997
Pages
817 - 820
Database
ISI
SICI code
0008-4301(1997)75:5<817:ASAECD>2.0.ZU;2-H
Abstract
DNA fingerprinting has become a powerful tool in behaviourial and evol utionary ecology. However, investigators have been limited in their ac cess to this technique because of the need for special radioisotope fa cilities. We present an alternative, non-isotopic DNA fingerprinting t echnique based on chemiluminescent detection of digoxigenin-labelled m inisatellite probes. This methodology should provide a greater number of researchers with access to DNA fingerprinting without the need for special laboratory facilities.