S. Amarri et al., C-13 and H-2 breath tests to study extent and site of starch digestion in children with cystic fibrosis, J PED GASTR, 29(3), 1999, pp. 327-331
Citations number
17
Categorie Soggetti
Pediatrics,"Medical Research General Topics
Journal title
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION
Background: Starch is an important source of energy for children with cysti
c fibrosis, but little is known about their capacity to digest it.
Methods: A C-13 breath test was used to measure starch digestion and oxidat
ion in 16 children with cystic fibrosis (median [range] age, 7.9 [4-15] yea
rs; 7 girls, 9 boys) and 5 normal healthy control subjects (median age, 8.3
[7-13] years; 3 girls, 2 boys). A test meal of C-13 flour and lactulose wa
s consumed and breath samples were obtained half-hourly thereafter for 6 ho
urs to measure C-13 enrichment by isotope ratio mass spectrometry and H-2 b
y electrochemistry. The test was repeated on 10 children with cystic fibros
is when they were taking pancreatic supplements.
Results: The median (range) cumulative percentage C-13 dose recovery (cPDR)
, was 35% (18-52%) in control subjects, 18% (9-33%) in children with cystic
fibrosis without enzymes, and 29% (22-51%) in those with pancreatic supple
ments, cPDR differed significantly between healthy control subjects and chi
ldren with cystic fibrosis without enzymes (p = 0.01) and between children
with cystic fibrosis with and without enzymes (p < 0.0001), but there was n
o difference between control subjects and children with cystic fibrosis tak
ing enzymes (p = 0.5). Eight children with cystic fibrosis had a cPDR withi
n control range,and in six there was a second peak in (CO2)-C-13 enrichment
coincident with an increase in H-2.
Conclusions: Starch digestion and oxidation are diminished in children with
cystic fibrosis, but pancreatic enzymes restored them to near normal level
s. A second peak in (CO2)-C-13 enrichment, suggestive of colonic starch fer
mentation was absent in healthy children, but present in some children with
cystic fibrosis and abolished by pancreatic enzymes.