Cell surface detection of HLA-E gene products with a specific monoclonal antibody

An HLA-E-specific monoclonal antibody (mAb) was obtained by immunization of human beta 2-microglobulin transgenic mice (M-TGM) with spleen cells from double transgenic mice expressing HLA-E molecules (EM-TGM). This mAb, desig nated V16, specifically recognizes in flow cytometry analysis the HLA-E exp ressing mouse cells, whereas it does not bind to mouse cells expressing var ious HLA class I molecules (HLA-A2, -A3, -A11, -A26, A29, -B7, -B27, -Cw3, -Cw7, and HLA-G). V16 mAb binds efficiently to human EBV-infected B lymphoc ytes, PHA blasts and PBL, thus establishing the surface expression of HLA-E in vivo on these cells. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.