Point of contact between synthetic chemistry and pharmacognosy: Monoclonalantibody against naturally occurring bioactive compounds and biosynthetic enzyme

MAb against THCA was produced by fusing hybridoma with splenocyts immunized with THCA-BSA conjugate and mouse myeloma cell. The cross-reaction of anti -THCA MAb against other marihuana compounds was very wide. This ELISA was a pplied to the biotransformation experiments of marihuana compounds using pl ant tissue culture. Anti-ginsenoside Rb1 MAb cross-reacted with only limited to a few ginsenosi des. Newly developed Western blotting used anti-ginsenoside Rb1 MAb serveye d only dammaran saponins. Immunoffinity column made it possible to separate ginsenoside Rb1 directly. Compact MAb, scFV against forskolin was prepared from a hybridoma by gene construction, and its expression in E. coli was d iscussed. Biosynthetic enzymes, THCA-, CBCA- and CBDA-synthase had been purified from the homogenate of fresh Cannabis plants, respectively. These characterizat ions were investigated and compared each other. It became evident that CBCA -synthase catalized enantiomeric cyclization of CBGA to CBCA.