Steric considerations in the covalent binding of myoglobin to thin films

Myoglobin is shown to bind to mixed thin films of docosyl mesylate and meth yl docosanoate. Myoglobin can be attached to films containing docosyl mesyl ate; however, films containing only methyl docosanoate exhibit only minimal myoglobin attachment. This indicates that myoglobin is specifically attach ed to the docosyl mesylate sites on the film surface. Surface coverage of m yoglobin is dependent on the percentage of docosyl mesylate in the film. A maximum myoglobin coverage of approximately 1.2 x 10(12) myoglobin/cm(2) is estimated for films composed of between 22% and 30% docosyl mesylate.