J. Jorgensen et al., ISOLATION AND CULTIVATION OF SPORE-FORMING FILAMENTOUS BACTERIA FROM PORCELLIO SCABER, Canadian journal of microbiology, 43(2), 1997, pp. 129-135
An endospore-forming rod-shaped filamentous bacterium was taken from b
oiled intestines of common sow bugs (Porcellio scaber, isopod crustace
ans). The bacteria were grown on peptone - yeast extract medium. As ma
ny as 180 cells per filament were counted in culture; filament length
was a function of time after germination and oxic conditions. Cultures
continued to grow filamentously after 10 successive transfers. The de
velopment of spores was inhibited by strict anaerobiosis for 3 months.
Spore-forming filaments over 100 mu m long in fresh intestinal materi
al were observed only in guts taken from sow bugs cultivated in darkne
ss. Phenotypic tests presented here show this isolate to be a member o
f the genus Bacillus, most closely resembling B. cereus.