Structural studies on the polar glycoinositol phospholipids of Trypanosoma(Schizotrypanum) dionisii from bats

The polar glycoinositol phospholipids (GIPLs) of a Trypanosoma species that belongs to the Schizotrypanum subgenus were purified by reversed-phase and normal-phase liquid chromatography and analysed by negative-ion mode elect rospray-mass spectrometry (ES-MS). The phosphatidylinositol moieties were r eleased by nitrous acid deamination and identified as ceramide- and alkylac ylglycerol-containing species. The structures of the GIPLs were determined using chemical treatments, sequential exoglycosidase digestions and positiv e-ion mode ES-MS-MS. All of the GIPLs were based on the same Man alpha 1-2M an alpha 1-2Man alpha 1-6Man alpha 1-4(NH2-CH2CH2-HPO3-)GlcN-PI core with s ingle terminal Galf residue substitutions either on the terminal nonreducin g Man or on the second alpha Man residue from the inositol and with either ethanolamine phosphate or 2-aminoethylphosphonate on the third alpha Man re sidue from the inositol. The T. (S.) dionisii GIPLs are compared with those of T. (S.) cruzi, a closely related species of the Schisotrypanum subgenus . (C) 1999 Elsevier Science B.V. All rights reserved.