Suppression of Ras-induced apoptosis by the Rac GTPase

Ras is an essential component of signal transduction pathways that control cell proliferation, differentiation, and survival. In this study we have ex amined the cellular responses to high-intensity Ras signaling, Expression o f increasing amounts of the oncogenic form of human HRas, HRasV12, results in a dose-dependent induction of apoptosis in both primary and immortalized cells. The induction of apoptosis by HRasV12 is blocked by activated Rac a nd potentiated by dominant interfering Rac. The ability of Rac to suppress Res-induced apoptosis is dependent on effector pathway(s) controlled by the insert region and is linked to the activation of NF-kappa B. The apoptotic effect of HRasV12 requires the activation of both the ERK and JNK mitogen- activated protein kinase cascade and is independent of p53. These results d emonstrate a role for Rac in controlling signals that are necessary for cel l survival, and suggest a mechanism by which Rac activity can confer growth advantage to cells transformed by the ras oncogene.