The role of beta-catenin stability in mutant PS1-associated apoptosis

MOST early onset cases of familial Alzheimer's disease (FAD) are caused by mutations in presenilin-1 (PS1) and presenilin-2 (PS2). These mutations lea d to increased beta-amyloid formation and induce apoptosis when expressed i n vitro. Recently, PS1 has been reported to associate with beta-catenin, an armadillo repeat protein. PS1 may regulate the function of beta-catenin, a nd mutant PS1 may disrupt this regulation. In the present study, we confirm that PS1-WT, as well as mutant PSI, associates with beta-catenin, and that mutant PS1 expression decreases the stability and/or enhances the degradat ion of beta-catenin. Most importantly, we correlate beta-catenin's destabil ization with mutant PS1-associated apoptosis by administering drugs that al ter the stability of beta-catenin. The application of LiCl and a proteasome inhibitor, N-acetyl-leu-leu-norleucinal (ALLN), increased the stability of cytosolic beta-catenin in mutant PS1-expressing cells leading to rescue of these cells from apoptosis. These studies suggest that beta-catenin is a k ey mediator of mutant PS1-associated apoptosis and FAD pathogenesis. NeuroR eport 10:2527-2532 (C) 1999 Lippincott Williams & Wilkins.