Genomic organization of the human X11L2 gene (APBA3), a third member of the X11 protein family interacting with Alzheimer's beta-amyloid precursor protein

RECENTLY we cloned a new member of the X11 protein family, X11L2 (gene symb ol APBA3) which interacts with Alzheimer's beta-amyloid precursor protein ( APP) and has three protein-protein interaction domains, a phosphotyrosine i nteracting domain (PID) and two PDZ. Here, we report the genomic structure and mapping of the APBA3. The gene spans about 11kb and is composed of ten coding exons and one untranslated exon. The transcription start site of APB A3 was found in a CpG island. About 1.2kb of the 5'-flanking region was als o sequenced, and its functional promoter activity was confirmed by transien t transfection experiments. The APBA3 was localized by the radiation hybrid mapping to chromosome 19. Determination of the APBA3 genome structure will facilitate the linkage analysis and search for mutations in the APBA3 in p atients with Alzheimer's disease. (C) 1999 Lippincott Williams & Wilkins.