Monomeric stabilization of insulin in solution

The primary condition necessary to achieve absorption of insulin via a muco us membrane is the prevention of association occurring. Transport which is not dependent on an enzymatic degradation of the molecule is only possible in monomeric form. Various substances such as alkylsaccharide, D-(+)-glucos e and urea were tested with respect to their effect on the prevention of a molecule association and on stability of the insulin molecule. It could be shown that the addition of the alkylsaccharide dodecyl-beta-D-maltosid caus es the desired monomeric distribution of the insulin molecules in a pH 7.4 buffer solution, and that this is also connected with a stabilization of th e molecule.