Structure-based design of an RNA-binding zinc finger

Citation
Dj. Mccoll et al., Structure-based design of an RNA-binding zinc finger, P NAS US, 96(17), 1999, pp. 9521-9526
Citations number
33
Categorie Soggetti
Multidisciplinary
Journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN journal
00278424 → ACNP
Volume
96
Issue
17
Year of publication
1999
Pages
9521 - 9526
Database
ISI
SICI code
0027-8424(19990817)96:17<9521:SDOARZ>2.0.ZU;2-Q
Abstract
A structure-based approach was used to design RNA-binding zinc fingers that recognize the HIV-1 Rev response element (RRE). An arginine-rich cu-helix from HIV-1 Rev was engineered into the zinc finger framework, and the desig ned fingers were shown to bind specifically to the RRE with high affinity a nd in a zinc-dependent manner, and display cobalt absorption and CD spectra characteristic of properly folded fingers. The results indicate that a mon omeric zinc finger can recognize a specific nucleic acid site and that the cu-helix of a finger can be used to recognize the major groove of RNA as we ll as DNA, The RRE-binding zinc fingers demonstrate how structure-based app roaches may be used in the design of potential RNA-binding therapeutics and provide a framework for selecting RNA-binding fingers with desired specifi cations.