T cell ignorance in mice to Borna disease virus can be overcome by peripheral expression of the viral nucleoprotein

Citation
J. Hausmann et al., T cell ignorance in mice to Borna disease virus can be overcome by peripheral expression of the viral nucleoprotein, P NAS US, 96(17), 1999, pp. 9769-9774
Citations number
45
Categorie Soggetti
Multidisciplinary
Journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN journal
00278424 → ACNP
Volume
96
Issue
17
Year of publication
1999
Pages
9769 - 9774
Database
ISI
SICI code
0027-8424(19990817)96:17<9769:TCIIMT>2.0.ZU;2-B
Abstract
Infection of neonates with Borna disease virus (BDV) induces severe meningo encephalitis and neurological disorder in wild-type but not in beta(2)-micr oglobulin-deficient mice of strain MRL (H-2(k)). Temporary in vivo depletio n of CD8(+) T cells delayed BDV-induced disease for several weeks. Depletio n of CD4(+) T cells had a similar beneficial effect, indicating that the BD V-induced neurological disorder in mice is a CD4(+) T cell-dependent immuno pathological process that is mediated by CD8(+) T cells. Lymphocytes prepa red from brains of diseased mice were mainly from the CD8(+) T cell subset. They showed up regulation of activation markers and exerted strong MHC I-r estricted cytotoxic activity against target cells expressing the BDV nucleo protein p40, Infection of B10.BR (H-2(k)) or congenic C57BL/10 (H-2(b)) mic e resulted in symptomless, lifelong persistence of BDV in the brain. Superi nfection with a recombinant vaccinia virus expressing BDV p40 but not with other vaccinia viruses induced severe neurological disease and encephalitis in persistently infected B10.BR mice but not in persistently infected C57B L/10 mice, indicating that the disease-inducing T cell response is restrict ed to the nucleoprotein of BDV in H-2(k) mice, Our results demonstrate that the cellular arm of the immune system may ignore the presence of a replica ting virus in the central nervous system until proper antigenic stimulation at a peripheral site triggers the antiviral response.