Prooligonucleotide metabolism in a crude cell extract followed by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

Authors
Lioux, T Lefebvre, I Vasseur, JJ Imbach, JL
Citation
T. Lioux et al., Prooligonucleotide metabolism in a crude cell extract followed by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry, RAP C MASS, 13(16), 1999, pp. 1645-1649
Citations number
16
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
ISSN journal
09514198 → ACNP
Volume
13
Issue
16
Year of publication
1999
Pages
1645 - 1649
Database
ISI
SICI code
0951-4198(1999)13:16<1645:PMIACC>2.0.ZU;2-B
Abstract
Using matrix-assisted laser desorption/ionisation time-of-flight mass spect rometry (MALDI-TOFMS) we studied the decomposition pathway of a prooligonuc leotide during incubation in a crude cell extract. This study, involving no sample processing, except drop dailysis to remove salts, led us to identif y more than thirty metabolites from several metabolic pathways. Copyright ( C) 1999 John Wiley & Sons, Ltd.