Distribution of the actin cytoskeleton during the cell cycle of Yarrowia lipolytica and the visualization of the tubulin cytoskeleton by immunofluorescence

Actin distribution was examined during the cell cycle of the dimorphic yeas t Yarrowia lipolytica, showing the correlation between bud growth, nuclear migration and rearrangement of the actin cytoskeleton. The results correspo nd with observations made in cells of Saccharomyces cerevisiae, S. uvarum a nd Candida albicans. Localization of actin was also determined in hyphal ce lls, where actin is stained predominantly in the tig and also at the septum of hyphae. The standard methods used for tubulin immunostaining in S. cere visiae and C. albicans cells were adapted for application in Y. lipolytica. Copyright (C) 1999 John Wiley & Sons, Ltd.