Sixteen dairy cows were used in a 2 x 4 factorial experiment, where two rap
eseed feeds (RSF) were compared at levels of 0, 1, 2 and 3 kg (fresh weight
) day(-1) replacing basal concentrate. The total amount of concentrate fed
was 10 kg day(-1) and restrictively fermented grass silage was fed ad libit
um. The experiment was arranged as a cyclic change-over design with four pe
riods of 3 weeks each. The experimental feeds were rapeseed meal (RSM) and
heat-moisture-treated rapeseed cake (RSC). Silage and total dry matter inta
ke, milk production and milk constituent outputs were linearly increased as
the proportion of RSF in the diet increased (at least P < 0.01). There wer
e no differences between RSM and RSC. Increased RSF intake led to a linear
increase (P < 0.001) in the concentrations of essential and branched-chain
amino acids in the blood plasma of the cows. Feeding RSC resulted in higher
concentrations of the essential amino acids histidine, isoleucine, leucine
and valine (at least P < 0.05), but the concentration of methionine tended
to be lower (P < 0.10) than on RSM diets. Utilization of amino acids absor
bed from the small intestine (AAT) was not affected by dietary treatments,
when feed table values, i.e. similar values for both RSFs, were used, but i
t was progressively increased for RSM diets when AAT values based on rumina
l and duodenal nylon bag incubations were used. This suggests that the nylo
n bag method underestimated the protein value of RSM.