Platelet-derived growth factor (PDGF-AB) like immune reactivity in serum and in cerebral spinal fluid following experimental subarachnoid haemorrhagein dogs

The aim of this study was to evaluate the following questions: Can the plat elet-derived growth factor (PDGF-AB) be identified in the serum and cerebro spinal fluid (CSF) of dogs? Is there an increase in the concentration of P DGF-AB following experimental subarachnoid haemorrhage (SAH)? Is the increa se in concentration related to the angiographic cerebral vasospasm of the b asilar artery. The "double haemorrhage" model was applied in seven dogs to produce experimental SAH with determination of angiographic vasospasm in th e basilar artery. Blood and CSF samples were taken on the first, third and eighth days. The analyses were performed with an ELISA human PDGF-AB antibo dy kit (quantikine human PDGF-AB, R&D Systems, Minneapolis, USA). The average PDGF-AB base value in the serum on the day before the SAH was 4 10.77 +/- 177.56 pg/ml, in the CSF it was 6.43 +/- 3.19 pg/ml. There was a significant (p = 0.05) increase in the concentration of PDGF-AB (third day 717.35 pg/ml, eighth day 918.07 pg/ml) in the serum of all animals. No sign ificant increase was found in the CSF samples of any animal. In summary, a PDGF-AB like immune reactivity was found in the serum of dogs with the huma n PDGF-AB ELISA kit and the concentration of PDGF-AB in the serum increased after experimental SAH but not in CSF, but there was no relationship betwe en the increase in PDGF-AB serum concentration and angiographic vasospasm.