Histological classification of Raman spectra of human coronary artery atherosclerosis using principal component analysis

We present a nonparametric method of analysis of Raman spectra of coronary artery tissue to classify atherosclerotic lesions. The method correlates th e principal component scores of the Raman spectra with the tissue pathology . A data set composed of 97 samples of human coronary artery was used to de velop the diagnostic algorithm, and a second data set composed of 68 sample s was then used to test this algorithm prospectively. The results show that the algorithm can accurately classify coronary artery tissue into three cl asses: nonatherosclerotic, noncalcified plaque, and calcified plaque. The a ccuracy of this classification scheme is comparable to that previously achi eved by means of a biochemical analysis of the Raman spectra using the same data.