By. Kang et al., Release of renal dipeptidase from rabbit renal proximal tubules and its inhibition by gentamicin, ARCH PH RES, 22(4), 1999, pp. 367-371
Effects of several drugs on rabbit renal proximal tubules were examined for
the applicability of renal dipeptidase (RDPase, EC 3. 4. 13. 11) release a
s a model system to study nephrotoxicity. The proximal tubule prepared by t
he method of Taub (1990) released RDPase spontaneously in the control exper
iment which was confirmed by Western blotting. RDPase was also released fro
m cisplatin, lipopolysaccharide (LPS), and indomethacin-treated tubules. Ge
ntamicin inhibited RDPase release in a concentration-dependent manner. This
RDPase release system may not be a general model to screen nephrotoxicity
but could be a useful source of RDPase purification in a simple and inexpen
sive way.