Anticomplement activities of oleanolic acid monodesmosides and bisdesmosides isolated from Tiarella polyphylla

Seven known oleanolic acid glycosides (1-7) were isolated from the MeOH ext ract of Tiarella polyphylla. The structures were identified to be 3-O-(beta -D-glucopyranosyl) oleanolic acid (1), 3-O-[beta-D-glucopyranosyl-(1 --> 3) -beta-D-glucopyranosyl] oleanolic acid (2), 3-O-[beta-D-glucopyranosyl- (1 --> 2)-beta-D-glucopyranosyl] oleanolic acid (3), 3-O-[beta-D-glucopyranosy l-(1 --> 3)-beta-D-glucopyranosyl] oleanolic acid 28-O-beta-D-glucopyranosy l eater (4), 3-O-[beta-D-glucopyranosyl-(1 --> 2)-beta-D-glucopyranosyl] ol eanolic acid 28-O-beta-D-glucopyranosyl ester (5), 3-O-[a-L-rhamnopyranosyl -(1 --> 3)-beta-D-glucuronopyranosyl] oleanolic acid (6), and 3-O-[alpha-L- rhamnopyranosyl-(1 --> 3)-beta-D-glucuronopyranosyl] oleanolic acid 28-O-be ta-D-glucopyranosyl ester (7) on the basis of physicochemical and spectral data. These triterpene glycosides were tested for the anticomplement activi ty and hemolytic activity. Bisdesmosidic saponins, 4, 5, and 7, showed anti complement activity; in contrast, monodesmosidic saponins, 1-3, and 6, show ed direct hemolytic activity Methyl esterified monodesmosidic saponins show ed anticomplement activity at a low concentration and hemolytic activity at a high concentration.