The significance of the homozygous CYP2A6 deletion on nicotine metabolism:A new genotyping method of CYP2A6 using a single PCR-RFLP

Citation
K. Kitagawa et al., The significance of the homozygous CYP2A6 deletion on nicotine metabolism:A new genotyping method of CYP2A6 using a single PCR-RFLP, BIOC BIOP R, 262(1), 1999, pp. 146-151
Citations number
31
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006291X → ACNP
Volume
262
Issue
1
Year of publication
1999
Pages
146 - 151
Database
ISI
SICI code
0006-291X(19990819)262:1<146:TSOTHC>2.0.ZU;2-Q
Abstract
A convenient and specific CYP2A6 genotyping method was developed in this st udy. This method consisting of a single PCR-RFLP is capable of resolving th e genotype into either CYP2A6*1 (wild type), CYP2A6*2, or CYP2A6*3. Among 2 52 Japanese persons genotyped, 241 were genotyped as the wild type, 1 as an unknown variant, and none as either CYP2A6*2 or CYP2A6*3. A homozygous del etion was found in the 10 remaining subjects. To clarify the metabolic sign ificance of this deletion in the whole human body, urinary cotinine, the pr incipal metabolite of nicotine, was analyzed subsequent to smoking. Cumulat ed urinary cotinine excretion in the homozygously CYP2A6-deleted individual s was about one-seventh compared to the control group (wild type). This stu dy provides a firm experimental basis for correlating genotypic characteriz ation of CYP2A6 with phenotypic expression of nicotine metabolism. (C) 1999 Academic Press.