Protective role of intraperitoneally administered vitamin E and selenium in rats anesthetized with enflurane

The aim of this investigation was to determine levels of liver vitamins A a nd E and blood biochemical and hematological parameters in the enflurane an esthesia of rats. Fifty adult male Wistar rats were used in this study All rats were randomly divided into five groups. The first and second groups we re used as the control and anesthesia control groups, respectively, and onl y the placebo was intraperitoneally injected. The third group was intraperi toneally administered with vitamin E (dl-alpha-tocopheryl acetate, 100 mg/k g body weight), the fourth group with Se (Na2SeO3 1.5 mg/kg body weight), a nd the fifth group with vitamin E and Se (dl-alpha-tocopheryl acetate, 100 mg/kg body weight + Na2SeO3 1.5 mg/kg body weight). This administration was done for three times with overday intervals and the second, third, forth, and fifth group rats were taken to enflurane anesthetise for 2 h. The liver vitamin E level was slightly lower in the anesthesia control grou p than in control group. However, the liver vitamin E content was significa ntly (p < 0.05 and p < 0.01) increased in vitamin E, Se, and combination gr oups, whereas the vitamin A level in Liver was not statistically different. In general, plasma levels of alanine aminotransferase, creatin kinase, tota l bilirubin, urea, red blood cell counts, packet cell volume, and hemoglobu lin values were significantly (p < 0.05 and p < 0.001) increased during the anesthesia and returned to near control values after the vitamin E plus se lenium injection. However, administration of vitamin E had less effect on t he hematological and biochemical parameters compared to that of selenium an d their combination with vitamin E. However, the white blood cell count and levels of alkaline phosphatase, aspartate aminotransferase, total choleste rol, triglycerides, total protein, and creatinine were not statistically in fluenced by the anesthesia. In conclusion, we observed that plasma levels of some enzymes and metabolit es were significantly increased in the enflurane anesthesia of rats, wherea s the liver vitamin E levels were slightly decreased. Therefore, we observe d that vitamin E and selenium have a protective effect against anesthesia c omplication, but the effect of selenium appears to be much greater than the vitamin E.