We present here the first evidence of the achievement of an effective prote
ction against pasteurellosis of 50 mg gilthead seabream using a toxoid-enri
ched whole-cell bacterin, commerzialized as DI vaccine, by dip or bath proc
edures. The relative percent survival (RPS) values were slightly higher by
the dip method (84-90) than by prolonged bath (77-80). On the basis of thes
e results, we recommend a first immunization when larvae are around 50 mg a
nd a booster immunization when fish reach 2 g body weight.