B. Romagnolo et al., Intestinal dysplasia and adenoma in transgenic mice after overexpression of an activated beta-catenin, CANCER RES, 59(16), 1999, pp. 3875-3879
Mutations in the adenomatous polyposis coil gene or activating mutations in
the beta-catenin gene itself are thought to be responsible for the excessi
ve beta-catenin signaling involved in intestinal carcinogenesis, We generat
ed transgenic mice that expressed large amounts of a NH2-terminally truncat
ed mutant beta-catenin (Delta N131 beta-catenin) in the intestine. These mi
ce had multifocal dysplastic lesions in the small intestine, reminiscent of
the early Lesions observed in the mouse models of familial adenomatous pol
yposis. The number of apoptotic cells in the villi of these transgenic mice
was 3-4-fold higher than in nontransgenic mice, Expression of the truncate
d beta-catenin mutant in the kidney led to the development of severe polycy
stic kidney disease,
Our findings support the concept that deregulation of the beta-catenin sign
aling pathway is the major oncogenic consequence of adenomatous polyposis c
oli mutations in intestinal neoplasia.