The synthesis and hydrolysis of ethyl acetate was monitored with a Raman sp
ectrometer equipped with a 785-nm laser, confocal optics, an optical fiber,
a holographic transmission grating, and a charge coupled device (CCD) dete
ctor. In the Raman spectra, the signals were seen to vary in proportion to
the concentrations of ethanol, acetic acid and ethyl acetate. First-order r
eaction, rate constants were determined for the hydrolysis reaction, under
different experimental conditions according to a full factorial experimenta
l design. The rate constants were determined either by using the peak heigh
t at one single wavelength, or by using score values from principal compone
nts analysis (PCA) or partial least squares (PLS). The rate constants deter
mined agree well with literature values. It is shown that the single wavele
ngth and the PCA/PLS approaches give similar reaction profiles, where the s
tandard normal variate (SNV) transformation was used for spectral pretreatm
ent. The use of Raman spectroscopy for reaction monitoring in opalescent mi
xtures is also shown and discussed. (C) 1999 Elsevier Science B.V. All righ
ts reserved.