Electrophoretic separation and characterisation of gliadin fractions from isolates and nanoparticulate drug delivery systems

Gliadin is a group of polymorphic proteins that are commonly used for the p reparation of many pharmaceutical and cosmetic products. The aim of this st udy was to develop an analytical HPCE procedure fbr the identification and quantification of gliadin samples. For this purpose, the HPCE buffer compos ition were optimised to improve the resolution of separations. Therefore, s everal buffer modifiers were tested for use with 50 mM phosphate buffer in HPCE separations. Twenty percent acetonitrile and 0.05 % hydroxypropylmethy lcellulose provided optimal resolution while maintaining excellent reproduc ibility. In conclusion, this method can be successfully applied to the eluc idation of the different gliadin fractions in protein isolates and nanopart iculate dosage forms.