N. Tedla et al., Phenotypic and functional characterization of lymphocytes derived from normal and HIV-1-infected human lymph nodes, CLIN EXP IM, 117(1), 1999, pp. 92-99
Lymph nodes are the major site of cell-to-cell transmission and replication
of HIV-1. Trafficking of CD4(+) T lymphocytes into lymph nodes provides a
continual supply of susceptible target lymphocytes, and conversely, recruit
ment of CD8(+) T lymphocytes may be critical for the host response that att
empts to control HIV-1 replication. The present study was undertaken as no
detailed assessment of lymphocyte subpopulations in HIV-1-infected lymph no
des has previously been reported. Peripheral blood and single-cell suspensi
ons prepared from lymph nodes of patients with HIV-1 and control subjects w
ere analysed using three-colour flow cytometry. Approximately 80% of the ly
mphocytes in control lymph nodes were CD3(+) T lymphocytes, of which over 6
5% were CD4(+). The majority of the CD4(+) and CD8(+) T lymphocytes obtaine
d from both lymph nodes and blood of control subjects were immunologically
naive (CD45RA(+)). By contrast, in HIV-1-infected patients there was a sign
ificant reduction in the proportion of CD4(+) T lymphocytes and an expansio
n of the CD8(+) T lymphocyte subset in both lymph nodes and peripheral bloo
d. Furthermore, a high proportion of these T lymphocytes displayed a marker
for immunological memory (CD45RO(+)). T lymphocytes derived from HIV-1-inf
ected lymph nodes also showed altered expression of the adhesion molecules,
L-selectin and very late antigen-4 (VLA-4), but not leucocyte function-ass
ociated antigen-1 (LFA-1). In an in vitro adhesion assay, lymphocytes from
HIV-1- infected nodes were significantly more adhesive than control lymphoc
ytes on fibronectin, as well as recombinant human intercellular adhesion mo
lecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) substrates
. This combination of altered lymphocyte subpopulations in the HIV-1-infect
ed lymph nodes, as well as enhanced adhesion phenotype and function, sugges
ts that T lymphocyte traffic to lymph nodes in HIV disease may be an import
ant determinant of pathogenesis.