Genetic and physical mapping of the dreher locus on mouse chromosome 1

Mutations in the mouse dreher (dr) gene cause skeletal defects, hyperactivi ty, abnormal gait, deafness, white belly spotting, and hypoplasia of Muller ian duct derivatives. To map dr to high resolution, we utilized two crosses . Initially, me analyzed an intersubspecific intercross to construct a deta iled genetic map of simple sequence length polymorphism markers within a 6. 3-cM region surrounding the dr locus. Subsequently, we analyzed a second in tersubspecific intercross segregating for the dr(6J) allele, which position ed dr within a 0.13-cM region between Rxrg and D1Mit370. A physical contig of BAC clones spanning the dr critical region was constructed, and eight po tential dr candidate genes mere excluded by genetic or physical mapping. To gether these results lay the foundation for positional cloning of the dr ge ne. (C) 1999 Academic Press.