A sequence-ready 840-kb PAC contig spanning the candidate tumor suppressorlocus DBC1 on human chromosome 9q32-q33

A putative tumor suppressor locus involved in bladder cancer has been mappe d to human chromosome 9q32-q33 and designated DBC1. Our previous microsatel lite-based deletion mapping study indicated that DBC1 was localized between D9S1848 and AFMA239XA9. We have constructed an 840-kb sequence-ready conti g composed of bacteriophage P1-derived artificial chromosomes (PACs), which encompasses DBC1. Clones were initially identified by screening a PAC libr ary with markers localized to the region by physical mapping, and subsequen tly PAC end probes were used to complete the contig. This contig contains a minimum tiling path of six PAC clones between D9S1848 and AFMA239XA9. Thre e expressed sequence tags (ESTs) were mapped to the DBC1 region by screenin g 24 ESTs mapped to the surrounding area by radiation hybrids. One represen ted the gene for DBCCR1, a known candidate for DBC1, and the other two were novel. This contig and preliminary expression map form the basis for the i dentification of the bladder cancer tumor suppressor gene in this region. ( C) 1999 Academic Press.