Bile acid patterns in meconium are influenced by cholestasis of pregnancy and not altered by ursodeoxycholic acid treatment

Background-Data on meconium bile acid composition in newborn babies of pati ents with intrahepatic cholestasis of pregnancy (ICP) are relatively scant, and changes that occur on ursodeoxycholic acid (UDCA) administration have not been evaluated. Aims-To investigate bile acid profiles in meconium of neonates from untreat ed and UDCA treated patients with ICP. Maternal serum bile acid composition was also analysed both at diagnosis and delivery to determine whether this influences the concentration and proportion of bile acids in the meconium. Patients/Methods-The population included eight healthy pregnant women and 1 6 patients with ICP, nine of which received UDCA (12.5-15.0 mg/kg body weig ht/day) for 15+/-4 days until parturition. Bile acids were assessed in the meconium by gas chromatography-mass spectrometry and in maternal serum by h igh performance liquid chromatography. Results-Total bile acid and cholic acid concentrations in the increased (p< 0.01) in newborns from patients with ICP (13.5 (5.1) and 8.4 (4.1) mu mol/g respectively; mean (SEM)) as compared with controls (2.0 (0.5) and 0.8 (0. 3) mu mol/g respectively), reflecting the total bile acid and cholic acid l evels in the maternal serum (r = 0.85 and r = 0.84, p<0.01). After UDCA adm inistration, total bile acid concentrations decreased in the mother (simila r to 3-fold, p<0.05) but not in the meconium. UDCA concentration in the mec onium showed only a 2-fold increase after treatment, despite the much great er increase in the maternal serum (p<0.01). Lithocholic acid concentration in the meconium was not increased by UDCA treatment. Conclusions-UDCA administration does not influence the concentration and pr oportion of bile acids in the meconium, which in turn are altered by ICP. M oreover, this beneficial treatment for the mother does not increase meconiu m levels of potentially toxic metabolites of UDCA such as lithocholic acid.