Effect of adenosine derivatives on in vitro thrombus formation induced by shear stress

Background and Objective. Shear-stress is considered to be the first event of platelet aggregation in vivo and platelet adhesion may be enhanced under pathologic conditions (e.g. arterial occlusion), Design and Methods. We wanted to test the effect of adenosine derivatives o n platelet aggregation induced by shear-stress. By increasing platelet cAMP adenosine derivatives inhibit platelet activation. This in turn leads to P -selectin (CD62P) exposure, which is known to play a fundamental role in th e binding of platelets to leukocytes, This gives rise to thrombus formation , Results. The levels of cAMP (pmol/mL) prior to and after treating blood wit h the following compounds were respectively: PGE1 4.67+/-0.29, 9.33+/-0.58; SP64 5.2+/-0.34, 6.83+/-0.52; 2-Cl-adenosine 5.83+/-0.58, 7.45+/-0.55; NEC A 7.00+/-2.29, 8.00+/-1.76, Interpretation and Conclusions, High shear rate was studied using a filtera ggregometer which could be a good test for analyzing what happens under phy siologic conditions compared to other systems in which platelet aggregation only occurs after adding aggregating agents (Born aggregometer), (C)1999, Ferrata Storti Foundation.