Transcript identification on the CLN5 region on chromosome 13q22

Our efforts to clone the CLN5 gene, mutated in a severe children's brain di sease, variant late infantile neuronal ceroid lipofuscinosis (vLINCL, MIM25 6731), resulted in large-scale sequencing of genomic clones flanking the cr itical chromosomal region on 13q22. Computational and traditional transcrip t identification analyses of the resulting sequence were used to identify t he disease gene. In addition to the identification of the CLN5 gene, this e ffort produced a large amount of genomic sequence data. Here, we report a t ranscription map of the 107 kb sequence in the CLN5 region, based on tradit ional and computational gene identification strategies. Several transcripts were identified in this sequence. Queries against the database of expresse d sequence tags proved to be the most powerful tool for gene identification from large-scale sequence.