Desorption of human serum albumin and human fibrinogen from the poly(3-hydroxybutyrate) surface

The protein desorption of human serum albumin and human fibrinogen from the surface of poly(3-hydroxybutyrate) films was studied using ATR-FTIR spectr oscopy. The diffusion model for reversible and irreversible sandwiched laye rs was confirmed. The reversible ratio (ratio of reversible adsorbed concen tration to irreversible adsorbed concentration as a function of time allows for a suggestion as to a kinetic model of the initial stage of thromb form ation. The parameters of adsorption/desorption for both proteins are compar ed. The reversible ratio of plasma protein adsorption is proposed as a quan titative criterion of thromb-resistance behavior for polymers in biomedicin e; namely, controlled drug release vehicles, artificial vessels, magistrals , reservoirs for blood storage, and surgical threads, especially. The mecha nism of interaction of protein molecules with poly(3hydroxybutyrate (PHB) m acromolecules is discussed. (C) 1999 John Wiley & Sons, Inc.