Insulin and exercise decrease glycogen synthase kinase-3 activity by different mechanisms in rat skeletal muscle

Glycogen synthase activity is increased in response to insulin and exercise in skeletal muscle. Part of the mechanism by which insulin stimulates glyc ogen synthesis may involve phosphorylation and activation of Akt, serine ph osphorylation and deactivation of glycogen synthase kinase-3 (GSK-3), leadi ng to dephosphorylation and activation of glycogen synthase, To study Akt a nd GSK-3 regulation in muscle, time course experiments on the effects of in sulin injection and treadmill running exercise were performed in hindlimb s keletal muscle from male rats, Both insulin and exercise increased glycogen synthase activity (%I-form) by 2-3-fold over basal. Insulin stimulation si gnificantly increased Akt phosphorylation and activity, whereas exercise ha d no effect. The time course of the insulin-stimulated increase in Akt was closely matched by GSK-3 alpha Ser(21) phosphorylation and a 40-60% decreas e in GSK-3 alpha and GSK-3 beta activity. Exercise also deactivated GSK-3 a lpha and beta activity by 40-60%. However, in contrast to the effects of in sulin, there was no change in Ser21 phosphorylation in response to exercise . Tyrosine dephosphorylation of GSK-3, another putative mechanism for GSK-3 deactivation, did not occur with insulin or exercise, These data suggest t he following: 1) GSK-3 is constitutively active and tyrosine phosphorylated under basal conditions in skeletal muscle, 2) both exercise and insulin ar e effective regulators of GSK-3 activity in vivo, 3) the insulin-induced de activation of GSK-3 occurs in response to increased Akt activity and GSK-3 serine phosphorylation, and 4) there is an Akt-independent mechanism for de activation of GSK-3 in skeletal muscle.