Spectral analysis of the porphyrins incorporation into human blood

The porphyrins seem to be the most effective photosensitizers in photodynam ic therapy of cancer. Monomers and dimers of sulfonated and nonsulfonated p orphyrins [5,10,15,20-tetra-phenyl-porphyrin, 5,10,15,20-tetranaphtyl-porph yrin (TNP), 5,10,15,20-tetra-p-sulfonato-phenyl-porphyrin (TS4PP) and 5,10, 15,20-tetra-psulfonato-naphtyl-porphyrin], are studied in this paper by mea ns of different spectral methods (UV-vis, fluorescence and polarization flu orescence). The porphyrins as TNP seem to be the most effective photosensit izer especially in a DMSO:water binary mixture of solvent. Monomer-dimer an d J-aggregation equilibria and the temperature dependence of TS4PP spectra are also studied in this paper. The incorporation of the forms of these por phyrins into blood cells is studied by means of the cytofluorimetric method . (C) 1999 Society of Photo-Optical Instrumentation Engineers. [S1083-3668( 99)01003-5].