Capillary electrophoretic analysis of recombinant human apolipoprotein E -Calibration mode of a protein reference material

Actual lack of standardization of serum apolipoprotein (apo)E measurements prevents intensive studies on the significance of apoE concentration in cli nical chemistry. For this purpose the use of standards calibrated against a common reference material is essential in order to obtain reliable results . The assignment of a certified value to this reference material involves t he use of an accurate and non immunological technique. We demonstrate here that capillary electrophoresis in sodium dodecyl sulfate containing gel (SD S-CGE) can be a method of choice. ApoE quantification was performed accordi ng to the peak area and a standard solution of purified apoAI. We obtained very close results to that obtained by HPLC of phenylalanine apoE content m easurement i.e., 81.6 mg/l versus 84.6 mg/l and concluded that the SDS-CGE analysis is an accurate and reliable method for the certification of an apo E reference material to be used in serum apoE concentration measurements. T he SDS-CGE combines advantages of SDS-polyacrylamide gel electrophoresis th igh resolving power, rapidity and tolerance of complex sample with that of quantitative HPLC analysis (accuracy, precision, linearity and speed). (C) 1999 Published by Elsevier Science B.V. All rights reserved.