Capillary electrochromatography of peptides in a microfabricated system

Reversed-phase liquid chromatography of tryptic peptides is shown in the ca pillary electrochromatography mode using microfabricated columns. Although selectivity is different, a mixture of tryptic peptides from ovalbumin appe ars to be as easily separated in the CEC as HPLC mode. The major difference between a separation in the microfabricated CEC column and conventional se parations in the HPLC mode is that separations are more readily achieved in the isocratic mode in the lower surface area microfabricated CEC columns. (C) 1999 Elsevier Science B.V. All rights reserved.