Secondary insults such as hypotension and hypoxia with head injury are asso
ciated with increased mortality and morbidity in comparison with head injur
y alone, in the present study the changes of brain thromboxane A(2) (TXA(2)
) and prostacyclin (PGI(2)) levels in a Marmarou's rodent model of diffuse
brain injury with hypotension and hypoxia were observed and the effect of d
iaspirin cross linked haemoglobin solution (DCLHb) were also investigated.
Thirty-two male Sprague-Dawley (SD) rats were randomized into four groups:
sham; head injury alone; head injury with secondary insults; and injury wit
h insults followed by DCLHb administration. The results showed that there w
ere no changes in TXB2 and 6-keto-PFG(1a) (metabolites of TXA(2) and PGI(2)
) levels in the injury alone group, while TXB2 levels in the secondary insu
lts group were elevated significantly; both TXB2 and 6-keto-PGF(ta) levels
in the injury with insults followed by DCLHb administration group were augm
ented significantly in comparison with the corresponding value of sham at 4
postimpact. The only increase in TXA, levels in the secondary insults rats
suggests that an imbalance in TXA(2)-PGI(2) production contributes to the
traumatic secondary processes, which include ischaemia and oedema. It is hy
pothesized that DCLHb may exert its protective properties through increasin
g PGI(2) production in injured brain by improving blood supply to injured b
lood vessels.