S. Ikegawa et al., Cloning and characterization of human and mouse PROSC (proline synthetase co-transcribed) genes, J HUM GENET, 44(5), 1999, pp. 337-342
Large-scale DNA sequencing, coupled with in silico gene trapping, is a robu
st approach to identifying unknown genes in selected genomic regions. Using
this approach we have isolated a novel human gene, PROSC (for proline synt
hetase co-transcribed [bacterial homolog]), from human chromosome 8p11.2, a
nd its mouse counterpart. The human PROSC gene spanned 17kb of genomic DNA;
its cDNA was 2530bp long, with 8 exons that included an open reading frame
of 825 bp (275 amino acids). The mouse cDNA (Prosc), 1995 bp long, was pre
dicted to encode 274 amino acids. PROSC is ubiquitously expressed in human
tissues and has been highly conserved among divergent species from bacteria
to mammals, suggesting its important cellular function. The gene product i
s likely to be a soluble cytoplasmic protein, but its function remains to b
e determined.