Sp. Adler et al., A canarypox vector expressing cytomegalovirus (CMV) glycoprotein B primes for antibody responses to a live attenuated CMV vaccine (Towne), J INFEC DIS, 180(3), 1999, pp. 843-846
To develop a vaccine against cytomegalovirus (CMV), a canarypox virus (ALVA
C) expressing CMV glycoprotein (gB) was evaluated alone or in combination w
ith a live, attenuated CMV vaccine (Towne), Three doses of 10(6.5) TCID50 o
f ALVAC-CMV(gB) induced very low neutralizing or ELISA antibodies in most s
eronegative adults. However, to determine whether ALVAC-CMV(gB) could prime
for antibody responses, 20 seronegative adults randomly received either 10
(6.8) TCID50 of ALVAC-CMV(gB) or 10(6.8) TCID50 of ALVAC-RG, expressing the
rabies glycoprotein, administered at 0 and 1 month, with all subjects rece
iving a dose of 10(3.5) pfu of the Towne vaccine at 90 days. For subjects p
rimed with ALVAC-CMV(gB), neutralizing titers and ELISA antibodies to CMV(g
B) developed sooner, were much higher, and persisted longer than for subjec
ts primed with ALVAC-RG. All vaccines were well tolerated. These results de
monstrate that ALVAC-CMV(gB) primes the immune system and suggest a combine
d-vaccine strategy to induce potentially protective levels of neutralizing
antibodies.