Hw. Lee et al., The effects of peripheral leukocytes on the hippocampal neuronal changes in transient global ischemia and unilateral cerebral hemispheric infarction, J KOR MED S, 14(3), 1999, pp. 304-314
The participation of activated leukocytes and subsequent production of chem
ical mediators has been well accepted in the pathophysiology of hypoxic-isc
hemic injury. This study was performed to see the effects of leukocytes on
hippocampal neuronal damage in transient global ischemia induced by 10-min
occlusion of bilateral common carotid arteries (CCAs) with reperfusion for
various times, and in complete unilateral ischemia induced by 24-hr ligatio
n of left CCA, Leukopenia was induced by intraperitoneal injection of cyclo
phosphamide for 4 days. The results showed that hippocampal neuronal damage
s were worse at 6-hr reperfusion in leukopenic experimental group than in t
he control group. In comparison, 24-hr and 3-day reperfusion leukopenic gro
ups showed less numbers of damaged neurons and milder changes. The 5-day re
perfusion group showed inconsistent changes, Unilateral CGA occlusion showe
d extensive infarction in 83.3% of gerbils in the control group, compared t
o 25% of gerbils in the experimental group (p<0.05). These results strongly
suggest that the number of peripheral leukocytes were closely related to t
he development of delayed neuronal damage of hippocampus in transient globa
l ischemia and the incidence of infarction induced by 24-hr unilateral CCA
ligation.