Synthesis, in vitro pharmacology, and molecular modeling of very potent tacrine-huperzine A hybrids as acetylcholinesterase inhibitors of potential interest for the treatment of Alzheimer's disease
P. Camps et al., Synthesis, in vitro pharmacology, and molecular modeling of very potent tacrine-huperzine A hybrids as acetylcholinesterase inhibitors of potential interest for the treatment of Alzheimer's disease, J MED CHEM, 42(17), 1999, pp. 3227-3242
Eleven new 12-amino-6,7,10,11-tetrahydro-7,11-methanocycloocta[b]quinoline
derivatives [tacrine (THA)-huperzine A hybrids, rac-21-31] have been synthe
sized as racemic mixtures and tested as acetylcholinesterase (AChE) inhibit
ors. For derivatives unsubstituted at the benzene ring, the highest activit
y was obtained for the g-ethyl. derivative rac-20, previously prepared by o
ur group. More bulky substituents at position 9 led to less active compound
s, although some of them [9-isopropyl (rac-22), 9-allyl (rac-23), and 9-phe
nyl (rac-26)] show activities similar to that of THA. Substitution at posit
ion 1 or 3 with methyl or fluorine atoms always led to more active compound
s. Among them, the highest activity was observed for the 3-fluoro-9-methyl
derivative rac-28 [about 15-fold more active than THA and about 9-fold more
active than (-)-huperzine A]. The activity of some THA-huperzine A hybrids
(rac-19, rac-20, rac-28, and rac-30), which were separated into their enan
tiomers by chiral medium-pressure liquid chromatography (chiral MPLC), usin
g microcrystalline cellulose triacetate as the chiral stationary phase, sho
wed the eutomer to be always the levorotatory enantiomer, their activity be
ing roughly double that of the corresponding racemic mixture, the distomer
being much less active. Also, the activity of some of these compounds inhib
iting butyrylcholinesterase (BChE) was tested. Most of them [rac-27-31, (-)
-28, and (-)-30], which are more active than (-)-huperzine A as AChE inhibi
tors, turned out to be quite selective for AChE, although not so selective
as (-)-huperzine A. Most of the tested compounds 19-31 proved to be much mo
re active than THA in reversing the neuromuscular blockade induced by d-tub
ocurarine. Molecular modeling of the interaction of these compounds with AC
hE from Torpedo californica showed them to interact as truly THA-huperzine
A hybrids: the 4-aminoquinoline subunit of (-)-19 occupies the same positio
n of the corresponding subunit in THA, while its bicyclo[3.3.1]nonadiene su
bstructure roughly occupies the same position of the corresponding substruc
ture in (-)-huperzine A, in agreement with the absolute configurations of(-
)-19 and (-)-huperzine A.