Characterization of a technique for rapid pharmacokinetic studies of multiple co-eluting compounds by LC/MS/MS

Citation
Xc. Tong et al., Characterization of a technique for rapid pharmacokinetic studies of multiple co-eluting compounds by LC/MS/MS, J PHARM B, 20(5), 1999, pp. 773-784
Citations number
9
Categorie Soggetti
Chemistry & Analysis
Journal title
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
ISSN journal
07317085 → ACNP
Volume
20
Issue
5
Year of publication
1999
Pages
773 - 784
Database
ISI
SICI code
0731-7085(199909)20:5<773:COATFR>2.0.ZU;2-M
Abstract
A method for rapid pharmacokinetic screening of multiple potential drug can didates has been developed. This technique. based on the ability of liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) to independ ently monitor multiple components, enables the quantification of substances which may or may not be chromatographically resolved. Our results indicate that the limit of quantitation and accuracy of this multiple-compound LC/M S/MRM quantitation method are comparable to a single-compound LC/MS/MRM qua ntitation method. No apparent ion suppression due to the existence of extra neous compounds in the analytical solution and biological matrix effect are observed in the range of the calibration curve. The issue of potential res idual molecule cross-tall; interference existing in the multiple-reaction m onitoring mode has been discussed. This multiple-compound LC/MS/MRM quantit ation method can be used for high throughput pharmacokinetic screening and to assay mixtures that have co-eluting analytes or similar m/z of precursor ;product ion pairs. (C) 1999 Elsevier Science B.V. All rights reserved.