Xc. Tong et al., Characterization of a technique for rapid pharmacokinetic studies of multiple co-eluting compounds by LC/MS/MS, J PHARM B, 20(5), 1999, pp. 773-784
A method for rapid pharmacokinetic screening of multiple potential drug can
didates has been developed. This technique. based on the ability of liquid
chromatography coupled with tandem mass spectrometry (LC/MS/MS) to independ
ently monitor multiple components, enables the quantification of substances
which may or may not be chromatographically resolved. Our results indicate
that the limit of quantitation and accuracy of this multiple-compound LC/M
S/MRM quantitation method are comparable to a single-compound LC/MS/MRM qua
ntitation method. No apparent ion suppression due to the existence of extra
neous compounds in the analytical solution and biological matrix effect are
observed in the range of the calibration curve. The issue of potential res
idual molecule cross-tall; interference existing in the multiple-reaction m
onitoring mode has been discussed. This multiple-compound LC/MS/MRM quantit
ation method can be used for high throughput pharmacokinetic screening and
to assay mixtures that have co-eluting analytes or similar m/z of precursor
;product ion pairs. (C) 1999 Elsevier Science B.V. All rights reserved.