Determination of nonvolatile components of heated soybean oils separated with high-efficiency mixed-bed polystyrene/divinylbenzene columns

Whole heated soybean oils and their polar fractions were analyzed for nonvo latile components by high-performance size-exclusion chromatography (HPSEC) with evaporative light scattering detection (ELSD). High molecular-weight (MW) polymer compounds with MW greater than or equal to trimer were efficie ntly separated with new 3-mu m mixed-bed styrene/divinylbenzene copolymer c olumns. Peaks of high MW polymer components in the new column system appear ed to be sharper and more symmetrical than those obtained with other column s. in the model systems studied, continuous addition of water to partially simulate frying conditions resulted in a significant increase (up to 30%) i n the polar lipid content of the heated oils evaluated. Due to relatively h igh concentrations of monomeric triglycerides (84.6-93.5%) present in the w hole unfractionated oils, small but erratic variations in the compositional distribution of components were observed in oils containing different amou nts of added water. On the other hand, HPSEC-ELSD analyses of the polar fra ctions (monomeric triglycerides, 25.4-62.6%) showed significant changes in the content and composition of nonvolatile components with the amount of wa ter added. In general, prolonged heating with increasing amounts of water a ccelerated hydrolysis and polymerization of heated soybean oils. Discrepanc ies in total polymeric materials obtained from HPSEC composition data for w hole oils and polar fractions are discussed in terms of nonuniformity in sa mple matrices, detection limitations for minor components, and a nonlinear ELSD response rationale.