Theoretical development and experimental evaluation of imaging models for differential-interference-contrast microscopy

Authors
Preza, C Snyder, DL Conchello, JA
Citation
C. Preza et al., Theoretical development and experimental evaluation of imaging models for differential-interference-contrast microscopy, J OPT SOC A, 16(9), 1999, pp. 2185-2199
Citations number
29
Categorie Soggetti
Apllied Physucs/Condensed Matter/Materiales Science","Optics & Acoustics
Journal title
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION
ISSN journal
10847529 → ACNP
Volume
16
Issue
9
Year of publication
1999
Pages
2185 - 2199
Database
ISI
SICI code
1084-7529(199909)16:9<2185:TDAEEO>2.0.ZU;2-E
Abstract
Imaging models for differential-interference-contrast (DIC) microscopy are presented. Two- and three-dimensional models for DIC imaging under partiall y coherent illumination were derived and tested by using phantom specimens viewed with several conventional DIC microscopes and quasi-monochromatic li ght. DIC images recorded with a CCD camera were compared with model predict ions that were generated by using theoretical point-spread functions, compu ter-generated phantoms, and estimated imaging parameters such as bias and s heer. Results show quantitative and qualitative agreement between model and data for several imaging conditions. (C) 1999 Optical Society of America [ S0740-3232(99)01509-4] OCIS codes: 110.2990, 180.6900, 110.6880.