Identification of glucosinolates in radish (Raphanus sativus L.) and cross-breeds of R-sativus L. x Brassica oleracea L. (Raphanobrassica) by LC-MS

The glucosinolate profiles in Raphanus sativus and new intergenetic as well as interspecific hybrids between Raphanus sativus and Brassica oleracea ha ve been investigated by HPLC-UV and LC-MS. Tentative identification based o n W-spectra and retention times were confirmed by the indivdual LC-MS measu rements. In this context three glucosinolates (glucoraphenin, glucoerysolin and 4-methylthiobutenyl glucosinolate), which are known for their nutritio nal and resistance value in the brassicaceae plants, but are not available as reference standards could be characterized by MS detection for the first time. The presented LC-MS analyses, performed in the API-ES positive mode, proved to be a very sensitive and selective method for the identification of unknown glucosinolates in plant material. Possible applications for bree ding research as well as for quality control purposes are discussed.