Antifungal biflavones from Cupressocyparis leylandii

From the leaves of Cupressocyparis leylandii (Cupressaceae) cupressuflavone , 4'-O-methylcupressuflavone, amentoflavone, 7-O-methylamentoflavone, 4"'-O -methylamentoflavone and hinokiflavone were isolated. H-1- and C-13-NMR dat a for 4'-O-methylcupressuflavone are given for the first time. The biflavon es from cultivar varieties of C. leylandii (Naylor's Blue, Castlewellan Gol d) were chromatographically (HPLC) compared. The antifungal activity of cup ressuflavone and 4'-O-methylcupressuflavone against Alternaria alternata, C ladosporium oxysporum, Fusarium culmorum and F. avenaceum was assayed.