Alternative methods to prepare immuno-enzymatic tracers

We developed an alternative and original method to generate immuno-enzymati c tracers, which avoid the difficulties characterizing chemical procedures. Our strategy consists in designing hybrid genes between the DNA ending for Various proteic antigens or antibody fragments and the gene encoding the b acterial alkaline phosphatase, then to produce them in the bacteria Escheri chia coli, Numerous recombinant tracers between proteic antigens having var iable sizes and structural complexities and the alkaline phosphatase were t hus obtained, Similarly, we built and produced scFv/ and/or Fab/PhoA hybrid s. All these recombinant compounds are fully bifunctionals, and susceptible to replace conventional immuno-enzymatic tracers.